Composite

Part:BBa_K1592025

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-09-09)

ptrp mutant3+RBS+GFP

Ptrp(BBa_K191007) is a promoter with the trp operator. It will be repressed by trpR (BBa_K588000) and LovTAP.

Note that the original trp operator contains two SpeI sites.

We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1(BBa_K1592020), ptrp mutant2(BBa_K1592021), and ptrp mutant3(BBa_K1592022).

Ptrp mutants.png

This composite is used to validating if the mutants would affect the function.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 756


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